In Situ Hybridization Kit (ISH), BCIP, BioAssay(TM)

In situ hybridization (ISH) is a powerful method for gene expression analysis. In Situ Hybridization Kit (ISH), BCIP, BioAssay(TM) provides the reagents, including hybridization buffer, NBT/BCIP color development solution and washing buffer solutions, for pre-hybridization, hybridization, and post hybridization treatments. Features - microRNA detection - Non-isotopic, colorimetric detection of gene expression - RNase-free, ISH-optimized reagents - Can be used with either oligonucleotide or ribonucleotide probes - Tested by Digoxingenin labeled oligonucleotide probes - Optimization of ISH assay conditions - ISH detection in tissue section and/or tissue microarray slides Applications - Diagnostic determination and clinical decision-making - Gene mutation studies - Protein expression and function analysis Description: In Situ Hybridization Kit (ISH), BCIP, BioAssay(TM) provides researchers a method doing in situ hybridization with non-radioactive probes. Compared with radioactive methods, the sensitivity of this kit is the same while the procedure is simplified. This kit is suitable for digoxingenin labled probes combined with an alkaline phosphatase color development system. Using this kit, all disadvantages of working with radioactive materials can be avoided. Quality Control: In order to ensure the quality of In Situ Hybridization Kit (ISH), BCIP, BioAssay, a pre-test procedure is performed for each lot of the kits. The positive result is detected with a digoxingenin labeled Has-mirRNA 122a cDNA probe on a previous positive frozen tissue panel slide. Kit Components: Prehybridization solution, 1x2ml Hybridization solution, 1x2ml NBT, 1x120ul BCIP, 1x60ul SSC Buffer (20X), 1x20ml Alkaline Phosphatase Buffer (10X), 1x20ml Anti-Dig antibody, 1x10ul Blocking Solution (100X), 1x100ul Storage and Stability: Store components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.