Insulin-Like Growth Factor I Receptor, beta (IGF1Rb) (BSA & Azide Free)

IGF-1R consists of a- and b-subunits, which are disulfide-linked in a b-a-a-b configuration in the mature receptor. The a-subunit is completely extracellular, while the b-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. \n\nMW of Antigen: 95kD \n\nEpitope: aa1323-1337 \n\nApplications and Recommended Dilutions: \nAffinity Purification of HYBRID and IGF-1 R2,3 (For conjugation, order Ab without BSA) \nImmunoprecipitation (Not optimum)1 \nWestern Blotting (Well-suited for b-subunit)2 (Ab 1-2ug/ml for 2hrs at RT) The optimal dilution for a specific application should be determined by the investigator. \n\nPositive Control: Placenta or breast carcinoma \n\nCellular Localization: Cell membrane\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-21MX

Size

100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See I7661-21M.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Soos MA, et. al. J Biol Chem 1992, 267(18):12955

Additional Info

Reacts only weakly with hybrids of Insulin Receptor and IGF-1R while it reacts very well.3 So, it has proved useful in the isolation of hybrid receptors.3 Species Crossreactivity: human and rat.