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Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)

Cat no: I7661-11E

Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)

Recombinant Human IGF-1 is a single, non-glycosylated, polypeptide chain containing 70aa and having a molecular weight of 7,655Da. The sequence of the first five N-terminal amino acids was determined to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. Contains less than 1% dimers and aggregates.\n \nSource: E. coli\n\nMolecular Weight: ~7.65kD\n\nEndotoxin: (same/less than)0.1 ng/ug\n\nActivity: \nThe ED50 as determined by the dose-dependent proliferation of murine Balb/c 3T3 cells (measured by H-thymidine uptake) is <1.0ng/ml, corresponding to a specific activity of 1x10U/mg. For most in vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-11E

Size

100ug

Applications

ELISA

Form

Supplied as a lyophilized powder from 50mM acetic acid. No preservative added. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml.

Purity

(same/more than)98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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