Insulin-Like Growth Factor I, Recombinant, Mouse (IGF1)

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. \n\nRecombinant murine IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kD. \n\nAmino Acid Sequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. \n\nBiological Activity:\nIGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB\\C 3T3 cells (measured by 3H-thymidine uptake) is (same/less than)1.0ng/ml, corresponding to a specific activity of 1x10e5 U/mg. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-10E

Size

10ug

Form

Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O, 0.1% HSA or BSA to (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 98% by RP-HPLC, SDS-PAGE.

References

1. Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro. Fabian D, Il'kova G, Czikkova S, Theriogenology 2004 Feb;61(4):745-55 2. Insulin-like growth factor-I governs submucosal growth and thickness in the newborn mouse ileum. Herman AC, Carlisle EM, Gordon PV, Pediatr Res 2004 Mar;55(3):507-13 3. Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin and selenium. Kurzawa R, Glabowski W, Brelik P, Reprod Biol 2002 Jul;2(2):143-62 4. Development of leydig cells in the insulin-like growth factor-I (igf-I) knockout mouse: effects of igf-I replacement and gonadotropic stimulation. Wang G, Hardy MP, Biol Reprod 2004 Mar;70(3):632-9 5. Insulin-like growth factor-I improves cerebellar dysfunction but does not prevent cerebellar neurodegeneration in the calcium channel mutant mouse, leaner. Nahm SS, Frank TC, Sepulvado JM, Neurobiol Dis 2003 Nov;14(2):157-65 6. Insulin-like growth factor I (IGF-I) regulates endocrine activity of the embryonic testis in the mouse. Villalpando I, Lopez-Olmos V, J Steroid Biochem Mol Biol 2003 Aug;86(2):151-8.