Insulin-Like Growth Factor I, Recombinant, Rat (IGF1)

Insulin-Like Growth Factor I, Recombinant, Rat (IGF1) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. \n\nBiological Activity: \nRat IGF-1 is fully biologically active when compared to standards. The ED50, was calculated 2 methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be (same/less than)30ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be (same/less than)10ng/ml.\n\nAA Sequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7661-09Q

Size

10ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

~95% Analysis by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Insulin-like growth factor I (IGF-I) and its receptor (IGF-1R) in the rat anterior pituitary.\nEur J Neurosci 2007 Jan;25(1):191-200\n2. [Soy isoflavones with supplemental calcium provide protection against the loss of bone mass and influence insulin-like growth factor (IGF)-I after ovariectomy in rat]\nZhonghua Yu Fang Yi Xue Za Zhi 2006 Sep;40(5):328-31\n3. The effect of insulin-like growth factor-I on hepatocyte apoptosis after bile duct ligation in rat.\nDig Dis Sci 2006 Dec;51(12):2220-4\n4. Short-term effects of IGF-I and estradiol on LH secretion from female rat gonadotrophs.\nGrowth Horm IGF Res 2006 Oct-Dec;16(5-6):357-64\n5. Short-term strength training and the expression of myostatin and IGF-I isoforms in rat muscle and tendon: differential effects of specific contraction types.\nJ Appl Physiol 2007 Feb;102(2):573-81\n6. Insulin-like growth factor-I is an important antiapoptotic factor for rat leydig cells during postnatal development.\nEndocrinology 2007 Jan;148(1):128-39