Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)

IRAP (also known as placental leucine aminopeptidase, PLAP; leucyl cystinyl aminopeptidase, LNPEP; oxytocinase, Otase; vesicle protein of 165kD or vp165) belongs to the peptidase M1 family. IRAP (mouse 966aa, rat 1025 aa; human 1025 aa, chromosome 5q14.2-q15), a zinc-containing metalloprotein, degrades peptide hormones such as oxytocin, vasopressin, and angiotensin III, and inactivates Met-enkephalin and dynorphin. It is a type 2 membrane protein and is secreted due to proteolytic processing. IRAP is glycosylated and alternatively spliced to at least 3 isoform (1-15 aa missing in isoform 2 and 1-20 missing in isoform 3). The N-terminus (1-110 aa) is located in the cytoplasm, followed by TM domain (111-131 aa); the C-terminus (132-1025 aa) is extracellular. The secreted form is cleaved at 154-155 aa (processed secreted form 155-1025 aa). IRAP is highly expressed in placenta, heart, kidney, and small intestine. In brain, only the membrane form is found. The brain form is found to be 140kD due to differential glycosylation.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 ECL.\nELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: I8601-01A.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8601

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1) Keeler SR et al (1995) JBC 270, 23612; masticl CC et al (1994) JBC 269, 6089; Chi N-W et al (2000) JBC 38437; Sbodio JI et al (2002) Biochem. J. 361, 451;

Additional Info

Recognizes rat IRAP. Species sequence homology: mouse-82% and human-76%.