Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18)

DNA sequences encoding the extracellular domain of human Integrin alpha M (Phe 17-Asn 1104; Accession# P11215) (Corbi, A.L., et al., 1988, J. Biol. Chem. 263:12403) with an acid tail at the C-terminus and the extracellular domain of human Integrin b2 (Gln 22-Asn 700; Accession# P05107) (Kishimoto, T. et al., 1987, Cell 48:681) with a basic tail at the C-terminus were cloned downstream of the human CD33 signal peptides and co-expressed in Chinese \nhamster ovary (CHO) cells. \n\nSpecifictity:\nThe 1088aa human aM/CD11b ECD shares 73-76% aa sequence identity with mouse, rat, bovine and canine aM while the 678 aa human b2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, sheep, and porcine b2.\n\nMolecular Mass: \nBased on N-terminal sequencing, the recombinant human Integrin aM subunit starts at Phe 17 and has a calculated molecular mass of ~124.7kD. The N-terminal sequence of the b2 subunit was blocked, suggesting that the b2 subunit may start at the predicted Gln 23 and has a calculated molecular mass of a~79kD. As a result of glycosylation, the recombinant proteins migrate as ~165 and 100kD protein bands in SDS-PAGE under reducing conditions.\n\nActivity: \nMeasured by the ability of the immobilized protein to support the adhesion of CHO cells transfected with human ICAM-1. When 5x10e4 cells/well are added to Integrin aMb2-coated plates (5ug/ml, 100ul/well), 70-90% of the cells will adhere after 1 hour at 37 degrees C in the presence of 1mM MnCl2.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7661-37Y1

Size

50ug

Form

Supplied as lyophillized powder from 50mM Tris, 0.15M sodium chloride, 1mM MgCl2, pH7.4. Reconstitute with sterile PBS to 0.1mg/ml.

Purity

~ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: (same/less than) 1EU /ug (LAL).

References

1. Corbi, A.L. et al., 1988, J. Biol. Chem. 263:12403. 2. Kishimoto, T. K. et al., 1987, Cell 48:681. 3. Muto, S. et al. ,1993, J. Clin. Immunol. 13:175. 4. Hynes, R. O., 2002, Cell 110:673. 5. Arnaout, M. A. et al., 2005, Annu. Rev. Cell Dev. Biol. 21:381. 6. Lee, J. et al., 1995, Cell 80:631. 7. Olvig, C. and T. A. Springer, 1998, Proc. Natl. Acad. Sci. USA 95:4870. 8. Xia, Y. et al., 2002, J. Immunol. 169:6417. 9. Shi, M. et al., 2005, J. Biol. Chem. 280:30586. 10. Shimaoka, M. et al., 2003, Immunity 19:391. 11. Beglova, N. et al., 2002, Nat. Struct. Biol. 9:282. 12. Ustinov, V. A. and E. F. Plow, 2005, Biochemistry 44:4357. 13. Ludwig, I. S. et al., 2006, Curr. Opin. Pharmacol. 6:1. 14. Mayadas, T. N. and X. Cullere, 2005, Trends Immunol. 26:388. 15. Kishimoto, T. K. et al., 1987, Cell 50:193