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Integrin alphaV, beta3

Cat no: I7661-37A

Integrin alphaV, beta3

Integrin alpha V beta 3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alpha V beta 3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described (1). It has tested negative for HIV & Hepatitis. \n\nApplications: \nSuitable for use in Electrophoresis and as an Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2.\n\nFunctional Activity: \nConfirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alpha V beta 3 integrin will also interact with other alpha V beta 3 binding proteins in an RGD-dependent fashion.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months..

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SPECIFICATIONS

Catalog Number

I7661-37A

Size

50ug

Applications

WB

Form

Supplied as a liquid in TBS, 100mM Octyl-b-D-glucopyranoside, 0.01% sodium azide. This format of alpha V beta 3 is appropriate for functional evaluations.

Purity

(same/more than) 95% by SDS-PAGE (silver stain)

References

1. Belkin, V.M., et al., J. Cell Biol. 111: 2159

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