Interferon beta 1 (beta 1a), Recombinant, Human (BSA Free) (IFNb1, IFNb1a)

One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma.\n\nRecombinant Human Interferon-Beta 1a is a single, glycosylated polypeptide chain containing one N-glycosylation site, 166 amino acids and having a molecular mass of ~23kD\n\nSequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.\n\nAccession Number: V00534\n\nSpecific Activity: ~1x10e8u/mg\n\nBiological Activity:\nInterferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in the cytopathic effect inhibition assay as described [Rubinstein, et al.. In this antiviral assay for interferon about 1u/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta (Hu-IFN-b) provided by the National Institutes of Health. Unit of activity was measured on VERO cells with vesicular stomatitis virus (VSV).\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

I7662-14Q

Size

100KU

Form

Supplied as a liquid 50mM acetic acid, 0.1% BSA.

Purity

Chromatographically purified ((same/more than) 95%). Endotoxin: (same/less than) 0.1ng/ug (IEU/ug).

References

1. Rubinstein, S., Familletti, P.C., Pestka, S., Convenient Assay for Interferons, J. Virol. 37: 755-758 (1981). 2. Familletti, P.C., Rubinstein, S., Pestka, S., A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon, in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394 (1981). 3. Pestka, S., Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119: 14-23 (1986).