Interferon beta 1 (beta 1b), Recombinant, Human (IFNb1, IFNb1b)

One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1. \n\nRecombinant Human IFN-beta 1b is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18.5kD. The Interferon beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu. \n\nBiological Activity:\nInterferon beta-1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32x10e6IU/mg.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized Interferon beta-1b in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-14S

Size

2ug

Form

Supplied as a lyophilized powder containing 0mg/ml HSA, 50mg/ml dextrose. REconstitute with sterile ddH2O to a concentration >0.1mg/ml.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Autoantibody synthesis in primary progressive multiple sclerosis patients treated with interferon beta-1b. Bitsch A, Dressel A, Bogumil T, Poser S, J Neurol 2004 Dec;251(12):1498-1501 2. Cost-utility of interferon beta(1b) in the treatment of patients with active relapsing-remitting or secondary progressive multiple sclerosis. Kobelt G, Jonsson L, Eur J Health Econ 2003 Mar;4(1):50-9 3. Longitudinal analyses of the effects of neutralizing antibodies on interferon beta-1b in relapsing/remitting multiple sclerosis. Deisenhammer F, Giovannoni G, Mult Scler 2004 Dec;10(6):713-4; author reply 715 4. Interferon beta-1b in secondary progressive MS: results from a 3-year controlled study. Panitch H, Miller A, Weinshenker B, Neurology 2004 Nov 23;63(10):1788-95 5. Interferon beta-1b in secondary progressive MS: a combined analysis of the two trials. Kappos L, Weinshenker B, Thompson AJ, McFarland H, Neurology 2004 Nov 23;63(10):1779-87 6. Interferon beta-1b modulates serum sVCAM-1 levels in primary progressive multiple sclerosis. Bitsch A, Bahner D, Elitok E, Tumani H, Acta Neurol Scand 2004 Dec;110(6):386-92.