Interferon gamma, Recombinant, Human (Immune Interferon, type II interferon, T cell interferon, MAF, IFNg)

IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells.IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins.This promotes the presentation of antigen to T-helper (CD4+) cells.IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response.Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.Human IFN-gamma species-specific and is biologically active only in human and primate cells.Recombinant human IFN-gamma is a 16.8kD protein containing 144 amino acid residues.\n\nBiological Activity:\nAssay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0ng/ml.\nAssay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ~0.05ng/ml, corresponding to a specific activity of ~2x10e7 units/mg.\n\nAA Sequence:\nMQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ\n\nCrossreactivity:\nMonkey\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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