Interferon tau, Recombinant, Ovine (IFNt)

Interferon-tau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. Interferon-tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN- tau (Recombinant Ovine IFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-g-induced MHC class II expression. Interferon tau was shown to downregulate IFN-gamma-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). Interferon- tau did not upregulate the expression of MHC class II on CVE. Interferon- tau also inhibited the replication of Theiler's virus in CVE.\n\nDescription: \nRecombinant Ovine Interferon-Tau produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of ~20kD. Recombinant Ovine IFN-Tau is purified by proprietary chromatographic techniques.\n\nAmino-Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nOvine IFN-Tau is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5.7 x 107 IU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Ovine Interferon-Tau.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280 nm using the absorbency value of 1.027 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Ovine IFN-Tau as a Reference Standard.\n\nGene: Name:\nIFNT1 \n\nSolubility: \nIt is recommended to reconstitute the lyophilized interferon-Tau in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Ovine Tau Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IFN-Tau should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.\n\n

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SPECIFICATIONS

Catalog Number

I7662-70A1

Size

2ug

Form

Supplied as a lyophilized powder in 0.5X PBS, pH 7.4.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Methods for studying interferon tau stimulated genes.\n\nMethods Mol Med 2006;122:367-80\n\n2. Regulation of the ovine interferon-tau gene by a blastocyst-specific transcription factor, Cdx2.\n\nMol Reprod Dev 2006 May;73(5):559-67\n\n3. Effect of interferon-tau administration on endometrium of nonpregnant ewes: a comparison with pregnant ewes.\n\nEndocrinology 2006 May;147(5):2127-37\n\n4. Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.\n\nReprod Nutr Dev 2006 Jan-Feb;46(1):97-104\n\n5. Solution behavior of a novel type 1 interferon, interferon-tau.\n\nJ Pharm Sci 2005 Dec;94(12):2703-15\n\n6. Identification of endometrial genes regulated by early pregnancy, progesterone, and interferon tau in the ovine uterus.\n\nBiol Reprod 2006 Feb;74(2):383-94