Interleukin 1 alpha, Human, BioAssay(TM) ELISA Kit (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

This Human Interleukin-1a ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-1a (IL-1a) concentrations in serum, plasma, cell culture supernatant, and other biological fluids\n\nIL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.\n\nThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1a present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody-enzyme specific for IL-1a is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound in the initial step. The color development is stopped and the intensity of the color is measured.\n\nIn order to measure the concentration of IL-1a in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-1a concentration (pg/ml). The concentration of IL-1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\nSensitivity:\nCalibrator Diluent I: 5.0pg/ml\nCalibrator Diluent II: 4.6pg/ml \n\nRange:\n15.6-500pg/ml\n\nKit Components:\nI7663-02B1:Microtiter Plate 1x96 wells\nI7663-02B2: IL-1a (Biotin) Mab 1x11ml\nI7663-02B3: Avidin (HRP) 1x14ml\n*I7663-02B4: IL-1a Standard 2x1 vial\nI7663-02B5: Calibrator Diluent I 1x22ml \nI7663-02B6: Calibrator Diluent II 1x22ml .\nI7663-02B7: Wash Buffer (20X) 1x60ml \nI7663-02B8:. Substrate A 1x10ml \nI7663-02B9: Substrate B 10ml \nI7663-02B10: Stop Solution 1x14ml \n\nStorage and Stability:\nStore at 4 degrees C. Store I7663-02B4: IL-1a Standard powder at 4 degrees C, liquid at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7663-02B

Size

96Tests

Applications

ELISA

References

1. Abbas, A.K., et al., (1987) Am. J. Pathol. 129: 26. 2. Aribia, M.B., et al., (1987) J. Immunol. 139: 443. 3. Cho, K.J., et al., (2005) Stem Cells. 23: 383. 4. Dayer, J.M., et al., (1986) J. Clin. Invest. 74: 645. 5. Dejuna, E., et al., (1987) Blood 69: 635. 6. Dinarello, C.A. and S.M. Wolff (1982) Am. J. Med. 72: 799. 7. Dinarello, C.A. (1988) Blood Purif. 6: 164. 8. Dinarello, C.A. (1988) FASEB J. 2: 108. 9. Dinarello, C.A. (1991) Blood 77: 1627. 10. Dinarello, C.A. and S.M. Wolff (1993) New Engl. J. Med. 328: 106. 11. Eastgate, J.A., et al., (1988) Lancet 2: 706. 12. Hudelist, G., et al., (2005) Hum. Reprod. Advance Access published online on March 3, 2005 13. Herrmann, F., et al., (1988) J. Clin. Invest. 81: 1415. 14. Kaye, J., et al., (1984) Lymphokine Res. 3: 175. 15. Kobayashi, Y., et al., (1990). Proc. Natl Acad. Sci. USA 87: 5548

Additional Info

For the Quantitative Determination of Human Interleukin 1a \n(IL-1a) Concentrations in Serum, Plasma, Cell Culture Supernatant, and Other Biological Fluids.