Interleukin 1 alpha, Recombinant, Human (Interleukin-1 alpha, IL-1 alpha, IL1-alpha, IL1A, IL-1A, Hematopoietin-1, IL1F1)

Source:\nRecombinant corresponding to 159aa of human Interleukin-1 alpha expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~18kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of mouse D10S cellsis less then 0.001ng/ml, corresponding to a Specific Activity of 1x10 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663B

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Human interleukin-1 alpha gene expression is regulated by Sp1 and a transcriptional repressor. Cytokine 2005 May 21;30(4):141-53. 2. Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha. Stem Cells 2005 Mar;23(3):383-91. 3. Effect of early luteal phase administration of a single dose mifepristone on immunohistochemical distribution of interleukin 1 alpha (IL-1 alpha) and transforming growth factor beta 1 (TGF-beta 1) in mid-luteal phase ovary of the rhesus monkey. Indian J Physiol Pharmacol 2003 Apr;47(2):221-4. 4. [Analysis on association between the polymorphisms in apolipoprotein E, interleukin-1 alpha genes and Alzheimer's disease in Chengdu area]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2004 Apr;21(2):176-8. 5. [Content of tumor necrosis factor alpha and interleukin 1-alpha in patients with acute purulent pleural cavity]. Klin Khir 2003 Dec;(12):26-8. 6. Lack of association between the interleukin-1 alpha (-889) polymorphism and early-onset Parkinson's disease. Neurosci Lett 2004 Apr 15;359(3):195-7.