Interleukin 1 alpha, Recombinant, Porcine (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta . In pheochromocytoma cells, NGF induces the synthesis of IL1.\n\nDescription: \nRecombinant Porice IL-1 alpha produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. Porcine IL-1a is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nIL-1a is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of D10S cells is < 0.03 ng/ml.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of IL-1alpha.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IL-1a as a Reference Standard.\n\nGene: \nName: IL1A\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Porcine IL-1 alpha in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Porcine IL-1a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Porcine IL-1alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I7663Q

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.\n\nJ Endod 2006 Mar;32(3):186-92\n\n2. Intracellular interleukin-1 receptor antagonist type 1 antagonizes the stimulatory effect of interleukin-1 alpha precursor on cell motility.\n\nCytokine 2005 Nov 3;32(3-4):163-70\n\n3. Human interleukin-1 alpha gene expression is regulated by Sp1 and a transcriptional repressor.\n\nCytokine 2005 May 21;30(4):141-53\n\n4. Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha.\n\nStem Cells 2005 Mar;23(3):383-91\n\n5. Effect of early luteal phase administration of a single dose mifepristone on immunohistochemical distribution of interleukin 1 alpha (IL-1 alpha) and transforming growth factor beta 1 (TGF-beta 1) in mid-luteal phase ovary of the rhesus monkey.\n\nIndian J Physiol Pharmacol 2003 Apr;47(2):221-4\n\n6. [Analysis on association between the polymorphisms in apolipoprotein E, interleukin-1 alpha genes and Alzheimer's disease in Chengdu area]\n\nZhonghua Yi Xue Yi Chuan Xue Za Zhi 2004 Apr;21(2):176-8