Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1 alpha is a 17.7 kD protein containing 155 amino acid residues.\n\nPurity: (same/more than) 98% by SDS-PAGE and HPLC analyses\n\nForm: The sterile ?ltered solution was lyophilized from 0.5x Tris buffer saline, pH 8.0.\n\nEndotoxin: (same/less than) 0.1ng/ug (1EU/ug)\n\nBiologicalпїЅActivity: The ED50 as determined by the dose-dependent proliferation of murine D10S cells is < 0.005ng/ml, corresponding to a speci?c activity of > 2 x 109units/mg.\n\nAAпїЅSequenceпїЅ: MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS\n\nStability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degrees C. Reconstituted IL-1 alpha should be stored in working aliquots at-20 degrees C.\n\nReconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1пїЅ1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degrees C for 1 week or-20 degrees C for future use.\n\nCountryпїЅofпїЅOriginпїЅ: USA

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