Interleukin 1 beta, Recombinant, Mouse (Interleukin-1 beta, IL-1 beta, Il1b, Il-1b)

Source:\nRecombinant corresponding to 153aa of mouse Interleukin-1 beta expressed in E.coli is a non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~17.5kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of mouse D10S cellsis less then 0.002ng/ml, corresponding to a Specific Activity of 5x109 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm 2. Analysis by RP-HPLC, using a standard solution of mouse IL-1 beta as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-17B

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. A novel cis-acting element required for lipopolysaccharide-induced transcription of the murine interleukin-1 beta gene. Mol Cell Biol 1995 Jan;15(1):112-9. 2. Upstream NFIL-6-like site located within a DNase I hypersensitivity region mediates LPS-induced transcription of the murine interleukin-1 beta gene. \nJ Immunol 1994 Jul 1;153(1):143-52. 3. An NFIL-6 sequence near the transcriptional initiation site is necessary for the lipopolysaccharide induction of murine interleukin-1 beta. DNA Cell Biol 1994 Jun;13(6):561-9. 4. Escherichia coli: derived murine interleukin-1 beta with N-terminus partially N alpha-acetylated. Protein Expr Purif 1993 Oct;4(5):499-502. 5. An ELISA assay for murine interleukin-1 beta. J Immunol Methods 1993 May 26;161(2):257-64. 6. The structure of murine interleukin-1 beta at 2.8 A resolution. J Struct Biol 1991 Oct;107(2):189-95.