Interleukin 1 beta, Recombinant, Rat (IL-1b)

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta . In pheochromocytoma cells, NGF induces the synthesis of IL-1.\n\nDescription: \nRecombinant Rat Interleukin-1 beta produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The Recombinant Rat IL-1b is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nThe ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Rat Interleukin-1 beta.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of Rat IL-1b as a Reference Standard.\n\nGene: \nName: Il1b\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Rat Interleukin-1b in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Rat IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-1 beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I7663-23A

Size

2ug

Form

Supplied as a lyophilized powder in 10mM sodium citrate, pH 3.0.

Purity

(same/more than) 98.0% as determined by: \n(a) Analysis by RP-HPLC. \n(b) Anion-exchange FPLC. \n(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

References

1. Crocetin Inhibits mRNA Expression for Tumor Necrosis Factor-{alpha}, Interleukin-1{beta}, and Inducible Nitric Oxide Synthase in Hemorrhagic Shock.\n\nJPEN J Parenter Enteral Nutr 2006 Jul-Aug;30(4):297-301\n\n2. Interleukin-1 beta contributes to the upregulation of kappa opioid receptor mrna in dorsal root ganglia in response to peripheral inflammation.\n\nNeuroscience 2006 Jun 16;\n\n3. MyD88 is a Key Mediator of Anorexia, But Not Weight Loss, Induced by Lipopolysaccharide (LPS) and Interleukin-1{beta} (IL1{beta}).\n\nEndocrinology 2006 Jun 15;\n\n4. Centrally administered interleukin-1 beta sensitizes to the central pressor action of angiotensin II.\n\nBrain Res 2006 Jun 8;\n\n5. CpG DNA modulates interleukin 1 beta-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells.\n\nRespir Res 2006 Jun 1;7(1):84\n\n6. Histone deacetylase inhibitors prevent exocytosis of interleukin-1{beta}-containing secretory lysosomes: role of microtubules.\n\nBlood 2006 May 9;