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Interleukin 1 Receptor Antagonist (IL-1Ra)

Cat no: I7663-25L

Interleukin 1 Receptor Antagonist (IL-1Ra)

Interleukin-1 Receptor Antagonist is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning ~400kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.\n\nRecombinant Human IL-1RA produced in E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17kD. \n\nSource: Escherichia coli\n\nPurity: ~98% by RP-HPLC and SDS-PAGE\n\nForm: Supplied as a sterile filtered, lyophilized powder. No additives. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to >0.1mg/ml.\n\nAmino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.\n\nBiological Activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells was found to be 0.5ng/ml. \n\nProtein Content: \nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.89 as the extinction

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SPECIFICATIONS

Catalog Number

I7663-25L

Size

20ug

Form

Supplied as a sterile filtered, lyophilized powder. No additives. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to >0.1mg/ml.

Purity

~98% by RP-HPLC and SDS-PAGE

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