Interleukin 1 Receptor Type I (IL-1RI)

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family. IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway. Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.\n\nApplications: \nSuitable for use in RIA, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nRIA: 1:8000 \nImmunoprecipitation: 1:400-1:800 use non-specific rabbit IgG for pre-cleaning.\nELISA: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7663-25G

Size

1ml

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Arend, W.P., M. Malyak, et al. (1994). J. Immunol 153: 4766-4774. 2. Colotta, F., S.K. Dower, et al. (1994). Immunol Today 15: 562-566. 3. Colotta, F., F. Re, et al. (1993). Science 261: 472-475. 4. Dower, S.K., J.M. Wignall, et al. (1989). J Immunol 142 (12) 4314-20. 5. McMahon, C.J., J.L. Slack, et al (1991). EMBO J 10: 2821-2832 6. Pruitt, J.H., M.B. Welborn, et al. (1996) Blood 87 (8): 3282-8 7. Symons, J.A., P.A. Young, et al (1993). Lymphokine Cytokine Res 12: 381. 8. Schreuder, H., C. Tardif, et al. (1997) Nature 386: 194-200. 9.Vigers, G. P., P. Caffes, et al. (1994) J. Biol. Chem. 269 (17): 12874-9.

Additional Info

Recognizes the extracellular domain of human IL-1R type I but precipitates the intact receptor expressed on human cells. Species Crossreactivity: primate Does not recognize human IL-1, IL-1Ra or IL-1R type II. Detects the extracellular domain of the IL-1R type I found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid in ELISA.