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Interleukin 10, Recombinant, Human (IL-10)

Cat no: I7663-07N

Interleukin 10, Recombinant, Human (IL-10)

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of a-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kD noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Ra/IL-10R1 chains, resulting in recruitment of two IL-10 Rb/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rb does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.\n\nBiological Activity: \nDetermined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be (same/less than) 2ng/ml, corresponding to a specific activity of (same/more than) 5 x 10e5 units/mg.\n\nEndotoxin: (same/less than)1EU/ug as determined by LAL method.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

I7663-07N

Size

2ug

Form

Supplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.

Purity

~95% by SDS-PAGE and HPLC analyses.

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