Sensitivity:
I8434-11E: Calibrator Diluent I <5pg/ml
I8434-11F: Calibrator Diluent II <5.1pg/ml
Range:
31.25-1000pg/ml
Specificity:
Recognizes both natural and recombinant human IL-12. No significant crossreactivity with human IL-1a, IL-b IL-2, IL-3, IL-4, IL-5 IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p40 IL-13 IL-15, GRO a GRO b, IFN g or mouse IL-12 p70 and IL-12 p40.
Kit Components:
I8434-11A: Microtiter Plate, 1x96 wells
I8434-11B: Pab (Biotin), 1x7ml
I8434-11C: Avidin (HRP). 1x14ml
I8434-11D: IL-12 Standard, 2x1 vial
I8434-11E: Calibrator Diluent I, 1x22ml
I8434-11F: Calibrator Diluent II, 1x22ml
I8434-11G: Wash Buffer (20X), 1x60ml
I8434-11H: Substrate A, 1x10ml
I8434-11J: Substrate B, 1x10ml
I8434-11K: Stop Solution, 1x14ml
Storage and Stability:
Store I8434-11D powder at 4 degrees C and liquid at -20 degrees C for 1 month. Store other components at 4C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.