Interleukin 13, Recombinant, Mouse (IL-13)

Interleukin-13 (IL-13) is a multifunctional cytokine whose net principle action is to diminish inflammatory responses. Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by human B cells. Previous studies showed that polymorphisms in the IL-13 gene were associated with serum total IgE or allergic asthma. Interleukin (IL)-13 is a pleiotropic cytokine, which shares many biological functions with IL-4. The receptor subunits of IL-13 consist of IL-4Ralpha, IL-13Ralpha1 and IL-13Ralpha2.\n\nDescription: \nRecombinant interleukin-13 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12127 Dalton. Mouse IL-13 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRecombinant IL-13 is fully biologically active when compared to standard. The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be < 4ng/ml, corresponding to a specific activity of >2.5 x 105.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of recombinant mouse IL-13.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of Interleukin-13 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Recombinant IL-13 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized IL-13 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-13 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8435-11B

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Interactions between leukotriene C4 and interleukin 13 signaling pathways in a mouse model of airway disease.\n\nArch Pathol Lab Med 2006 Apr;130(4):440-6\n\n2. Visceral Leishmania donovani infection in interleukin-13-/- mice.\n\nInfect Immun 2006 Apr;74(4):2487-90\n\n3. Role of early growth response-1 (Egr-1) in interleukin-13-induced inflammation and remodeling.\n\nJ Biol Chem 2006 Mar 24;281(12):8161-8\n\n4. Interleukin-16 inhibits interleukin-13 production by allergen-stimulated blood mononuclear cells.\n\nImmunology 2006 Jan;117(1):89-96\n\n5. Characterization of a novel PMA-inducible pathway of interleukin-13 gene expression in T cells.\n\nImmunology 2006 Jan;117(1):29-37\n\n6. Interleukin-13 is involved in the survival of children with sepsis.\n\nActa Paediatr 2005 Dec;94(12):1828-31