Interleukin 15, Recombinant, Mouse (IL-15)

Interleukin (IL)-15 is a pleiotropic pro-inflammatory cytokine that is expressed in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. IL-15 promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. Recent emerging data suggest that IL-15 may serve as a useful therapeutic target across a range of disease states. Advances in the past year highlight the beneficial effect of IL-15 neutralisation in models of psoriasis and diabetes. Further evidence for IL-15 expression and effector function has emerged across a range of rheumatic disorders, including juvenile inflammatory arthritis, rheumatoid arthritis and Kawasaki disease. Interleukin-15 (IL-15) is a gamma-common cytokine, which plays an important role in the development, survival and proliferation of NK, NK T and CD8+ T cells. \n\nRecombinant Murine IL-15 is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13321 Dalton. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Ile-Asp. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of mouse Interleukin-15. \n\nBiological Activity:\nrmIL-15 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 was found to be < 5ng/ml, corresponding to a Specific Activity of 2 x 10e5 IU/mg. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). . \n2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. \n\nReconstitution:\nReconstitute the lyophilized murine IL-15 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8437-05A

Size

2ug

Form

Supplied as a lyophilized powder in 10mM Tris, pH 8.0.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Failed adoptive immunotherapy with tumor-specific T cells: reversal with low-dose interleukin 15 but not low-dose interleukin 2. Roychowdhury S, May KF Jr, Lin T, Ferketich AK, Cancer Res 2004 Nov 1;64(21):8062-7 2. Interleukin-15-deficient mice develop protective immunity to Toxoplasma gondii. Lieberman LA, Villegas EN, Infect Immun 2004 Nov;72(11):6729-32 3. Interleukin-15 is not required for the induction or maintenance of orally induced peripheral tolerance. Millington OR, Wei XQ, Mowat AM, Immunology 2004 Nov;113(3):304-9 4. Synthesis and secretion of interleukin-15 by freshly isolated human bronchial epithelial cells. Ge N, Nishioka Y, Okano Y, Yanagawa H, Int Arch Allergy Immunol 2004 Nov;135(3):235-42 5. Serum levels of cancer antigen 125 and interleukin-15 in relation to the nutrition status of peritoneal dialysis patients. Grzegorzewska AE, Mlot M, Adv Perit Dial 2004;20:185-9 6. Effects of megestrol acetate on circulating interleukin-15 and interleukin-18 concentrations in healthy elderly men. Lambert CP, Flynn MG, Evans WJ, J Gerontol A Biol Sci Med Sci 2004 Aug;59(8):855-8.