Interleukin 15, Recombinant, Porcine (IL-15)

Interleukin (IL)-15 is a pleiotropic pro-inflammatory cytokine that is expressed in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. IL-15 promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. Recent emerging data suggest that IL-15 may serve as a useful therapeutic target across a range of disease states. Advances in the past year highlight the beneficial effect of IL-15 neutralisation in models of psoriasis and diabetes. Further evidence for IL-15 expression and effector function has emerged across a range of rheumatic disorders, including juvenile inflammatory arthritis, rheumatoid arthritis and Kawasaki disease. Interleukin-15 (IL-15) is a gamma-common cytokine, which plays an important role in the development, survival and proliferation of NK, NK T and CD8+ T cells.\n\nDescription: \nRecombinant Porcine IL-15 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 13,118 Dalton. Porcine IL-15 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Trp-Gln-His-Val.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nProcine IL-15 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 was found to be < 0.2 ng/ml.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280 nm using the absorbency value of 0.638 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Procine IL-15.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Procine IL-15 in sterile 18MO-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Procine IL-15 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Procine IL-15 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8437-05R

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Polymorphisms within interleukin 15 are associated with juvenile idiopathic arthritis.\n\nClin Exp Rheumatol 2006 Mar-Apr;24(2):219\n\n2. Interleukin-15 mediates protection against experimental tuberculosis: a role for NKG2D-dependent effector mechanisms of CD8+ T cells.\n\nEur J Immunol 2006 May;36(5):1156-67\n\n3. Peripheral blood T lymphocytes from patients with early rheumatoid arthritis express RANKL and interleukin-15 on the cell surface and promote osteoclastogenesis in autologous monocytes.\n\nArthritis Rheum 2006 Apr;54(4):1151-64\n\n4. The interleukin-15/interleukin-15 receptor system as a model for juxtacrine and reverse signaling.\n\nBioessays 2006 Apr;28(4):362-77\n\n5. Interleukin 15: its role in intestinal inflammation.\n\nGut 2006 Apr;55(4):444-5\n\n6. [Levels of serum interleukin-15 and the expression of T-helper lymphocyte subsets in peripheral blood of children with juvenile rheumatoid arthritis]\n\nZhongguo Dang Dai Er Ke Za Zhi 2006 Feb;8(1):9-12