Interleukin 17, Recombinant, Mouse (IL-17)

IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation\n\nDescription: \nRecombinant Murine IL-17 produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 266 amino acids and having a molecular mass of 29,956 Dalton. Recombinant Mouse Interleukin-17 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ala-Ile-Ile-Pro.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nMouse IL-17 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent secretion of IL-6 by NIH 3T3 cells was found to be 1.0-10.0 ng/mL,\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of mouse IL-17.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.942 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Recombinant Interleukin-17 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Mouse Interleukin-17 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Mouse Interleukin-17 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8439-05N

Size

5ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Interleukin-17 acts independently of TNF-alpha under arthritic conditions.\n\nJ Immunol 2006 May 15;176(10):6262-9\n\n2. Determination and characterization of bovine interleukin-17 cDNA.\n\nJ Interferon Cytokine Res 2006 Mar;26(3):141-9\n\n3. The effect of interleukin-17 on the proliferation and invasion of JEG-3 human choriocarcinoma cells.\n\nAm J Reprod Immunol 2006 Apr;55(4):291-300\n\n4. Association analysis of the interleukin 17 genes IL17A and IL17F as potential osteoarthritis susceptibility loci.\n\nAnn Rheum Dis 2006 Apr;65(4):556-7\n\n5. Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization.\n\nWorld J Gastroenterol 2005 Oct 28;11(40):6305-11\n\n6. Expression of interleukin-17 in ischemic brain tissue.\n\nScand J Immunol 2005 Nov;62(5):481-6