Interleukin 17A/F Heterodimer, Recombinant, Mouse (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8)

Source: \nTwo DNA sequences encoding the mature mouse IL-17A (Thr 22-Ala 158; Accession #Q62386) and mature mouse IL-17F (Arg 21-Ala 153; Accession # AAM77568) were expressed separately in E. coli. \n\nMolecular Mass: \nThe recombinant mouse IL1-7A/IL-17F is a refolded disulfide-linked heterodimer of IL-17A and \nIL-17F. The non-methionyl form of rhIL-17A monomer has a predicted molecular mass of 14.9kD whereas the methionyl form of rhIL1-7F monomer has a predicted molecular mass of 15.5kD.\n\nActivity: Measured by its ability to stimulate induce IL-6 production by NIH/3T3 cells (Yao, Z. et al., 1995, Immunity 3:811). The ED 50 for this effect is typically 0.075-0.375ug/ml.\n\nEndotoxin: (same/less than)1.0 EU/1ug (LAL)\n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8439-02M

Size

25ug

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in 30% acetonitrile, 0.1% TFA, 50ug of bBSA per 1ug of cytokine. Reconstitute with sterile 4mM HCI containing at least 0.1% HSA or BSA be added to the vial to prepare a stock solution of 100ug/ml.

Purity

(same/more than) 95% by SDS

References

1. Wright, J.F. et al., 2007, J. Biol. Chem. 282:13447. 7. Ouyang, W. et al., 2008, Immunity 28:454. 2. Kawaguchi, M. et al., 2004, J. Allergy Clin. Immunol. 114:1265. 8. Steinman, L., 2007, Nat. Med. 13:139. \n3. Kolls, J.K. and A. Linden, 2004, Immunity 21:467. 9. Hunter, C.A., 2005, Nat. Rev. Immunol. 5:521. 4. Moseley, T.A. et al., 2003, Cytokine Growth Factor Rev. 14:155. 10. Kuestner, R.E. et al., 2007, J. Immunol. 179:5462. 5. Cheung, P.F.Y. et al., 2008, J. Immunol. 180:5625. 11. Chang, S.H. and C. Dong, 2007, Cell Res. 17:435. 6. Liang, S.C. et al., 2007, J. Immunol. 179:7791. 12. Wright, J.F. et al., 2008, J. Immunol. 181:2799.