Interleukin 18, Rat, BioAssay(TM) ELISA Kit (IL18, IL-18, Interferon Gamma-inducing Factor, IFN-gamma-inducing Factor, Interleukin-1 Gamma, IL-1 Gamma, Il18, Igif)

The rat Interleukin 18 (IL-18) BioAssay(TM) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated rat IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-18 present in the samples. \n\nIntended Use:\nThe purpose of this kit is the in-vitro quantitative determination of rat IL-18 in samples such as sera, plasma, tissue lysates and cell culture supernates. This BioAssay(TM) kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.\n\nSensitivity:\n<1pg/ml\n\nRange:\n15.6pg/ml-1000pg/ml\n\nKit Components:\nLyophilized recombinant Rat Interleukin 18 standard (Reagent A), 4x10ng/tube\nPlate pre-coated with anti-rat Interleukin 18 antibody, 2x96wells\nSample diluent buffer, 2x30ml\nBiotinylated anti-rat Interleukin 18 (Reagent B), 2x130ul, dilution 1:100\nAntibody diluent buffer, 2x12ml\nAvidin-Biotin-Peroxidase Complex (ABC), 2x130ul, dilution 1:100\nABC diluent buffer, 2x12ml\nTMB color developing agent, 2x10ml\nTMB stop solution, 2x10ml\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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