Interleukin 18, Recombinant, Rat (IL-18, IFN-gamma inducing factor, IGIF)

Source: \nRecombinant corresponding to rat IL-18 expressed in E. coli.\n\nMolecular Weight: \n~18kD\n\nActivity:\nED50 range =3-10ng/ml, determined by the dose dependent secretion of interferon-gamma by activated mouse splenocytes. The optimal concentration for each specific application should be determined by an initial dose-response assay. \n\nEndotoxin: ~0.1ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8439-95R

Size

10ug

Form

Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Conti, B., J.W. Jahng, C. Tinti, J.H. Son, and T.H. Soh (1997) Induction of interferon-gamma inducing factor in the adrenal cortex. J. Biol. Chem. 272:2035-2037. 2. Dinarello, C.A., D. Novick, A.J. Puren, G. Fantuzzi, L. Shapiro, H. Muhl, D.Y. Yoon, L.L. Reznikov, S.H. Kim, and M. Rubinstein (1998) Overview of interleukin-18: more than an interferon-gamma inducing factor. J. Leukoc. Biol. 63:658-664. 3. Yoshimoto, T., K. Takeda, T. Tanaka, K. Ohkusu, S.-I. Kashiwamura, H. Okamura, S. Akira, and K. Nakanishi (1998) IL-12 upregulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-g production. J. Immunol. 161:3400-3407. 4. Krook, H., J. Wallstrom, and S. Sandler (1999) Function of rat pancreatic islets exposed to interleukin-18 in vitro. Autoimmunity 29:263-267. 5. Hegardt, P., B. Widegren, L. Li, B. Sjogren, C. Kjellman, I. Sur, and H.O. Sjogren (2001) Nitric oxide synthase inhibitor and IL-18 enhance the anti-tumor immune response of rats carrying an intrahepatic colon carcinoma. Cancer Immunol. Immunother. 50:491-501. 6. Curran, B., and J.J. O