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Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)

Cat no: I7663-28A1

Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. \n\nRecombinant Human IL-2 is a single non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134aa and having a molecular weight of 15.5kD. The sequence of the first five N-terminal aa was determined to be Met-Ala-Pro-Thr-Ser. Recombinant Human IL-2 has a Ser substitute for Cysteine at position 126. \n \nMolecular Weight: ~15.5kD\n\nActivity: \nThe ED50 as determined by the dose-dependent stimulation of mouse CTLL-2 cells is less than 0.0645ng/ml, corresponding to a specific activity of 16.9MIU/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-28A1

Size

50ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS, pH 7.5. No preservative added. Reconstitute with 20mM acetic acid to a concentration (same/more than)100ug/ml.

Purity

(same/more than) 98% (RP-HPLC, SDS-PAGE).

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