Interleukin 2, Recombinant, Mouse (IL-2)

Recombinant Murine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton. The Recombinant Murine IL-2 is purified by proprietary chromatographic techniques.\n\nAA Sequence: \nPTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ\n\nBiological Activity:\nRecombinant Murine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5 MIU/mg.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7663-26A1

Size

5ug

Form

Supplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0.

Purity

(same/more than) 98% as determined by RP-HPLC, SDS-PAGE.

References

1. Combination nonviral murine interleukin 2 and interleukin 12 gene therapy and radiotherapy for head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 2005 Dec;131(12):1079-85 2. Engineering of the gut commensal bacterium Bacteroides ovatus to produce and secrete biologically active murine interleukin-2 in response to xylan. J Appl Microbiol 2005;98(5):1191-7 3. [Effects of entrapment of murine interleukin-2 gene with chitosan nanoparticles on expression of mIL-2 gene and on regulation of immune response in mice] Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2004 Dec;21(6):947-52 4. Permeability of the mouse blood-brain barrier to murine interleukin-2: predominance of a saturable efflux system. Brain Behav Immun 2004 Sep;18(5):434-42 5. Effective genetic therapy of established medullary thyroid carcinomas with murine interleukin-2: dissemination and cytotoxicity studies in a rat tumor model. Endocrinology 1999 May;140(5):2152-8\n6. Genetic immunotherapy for medullary thyroid carcinoma: destruction of tumors in mice by in vivo delivery of adenoviral vector transducing the murine interleukin-2 gene. Thyroid 1998 Dec;8(12):1137-46