Interleukin 2, Recombinant, Rat (IL-2)

Recombinant Rat IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15311 Dalton. The Recombinant Rat IL-2 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRat IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.4 ng/ml, corresponding to a Specific Activity of 2.5 MIU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Rat IL-2.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.\n\nGene: \nName: IL2\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Recombinant Rat IL-2 in sterile 20mM AcOH not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Rat IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I7663-28B

Size

5ug

Form

Supplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Secretory production of biologically active rat interleukin-2 by Clostridium acetobutylicum DSM792 as a tool for anti-tumor treatment.\n\nFEMS Microbiol Lett 2005 May 1;246(1):67-73\n\n2. Functional coupling of NKR-P1 receptors to various heterotrimeric G proteins in rat interleukin-2-activated natural killer cells.\n\nJ Biol Chem 1997 Dec 12;272(50):31604-8\n\n3. Non-granular proteolytic enzymes of rat interleukin-2-activated natural killer cells. III. Enhancement of A-NKP 1, 2, and 3 proteolytic activities (cleaving after Arg, Phe and Pro) in response to interleukin-2.\n\nNat Immun 1995 Sep;14(5-6):286-94\n\n4. Rat interleukin-2 immunoglobulin M fusion proteins are cytotoxic in vitro for cells expressing the IL-2 receptor and can abolish cell-mediated immunity in vivo.\n\nTransplantation 1994 Oct 27;58(8):932-9\n\n5. Zymographic analysis of extracellular, released and cell-associated proteases of rat interleukin 2-activated natural killer (A-NK) cells.\n\nIn Vivo 1994 Jan-Feb;8(1):33-41\n\n6. Generation of a potentially immunosuppressive rat interleukin-2 immunoglobulin M fusion molecule.\n\nTransplant Proc 1993 Feb;25(1 Pt 1):774-5