Interleukin 20, Recombinant, Human (IL-20)

The newly discovered cytokine interleukin-20 (IL-20) is structurally related to IL-10, yet it appears to be an autocrine factor for keratinocytes that regulates their participation in inflammation. \n\nRecombinant Human IL-20 is a single, non-glycosylated homodimeric polypeptide chain containing 2x153 amino acids and having a total molecular mass of 35,212D. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\nIL-20 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human MO7e cells is < 0.2ng/ml, corresponding to a Specific Activity of 5x10e6 IU/mg. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of hIL-20. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of IL-20 as a Reference Standard. \n\nReconstitution:\nReconstitute the lyophilized Interleukin-20 in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8441-52

Size

2ug

Form

Supplied as a lyophilized powder in 100mM sodium chloride, 10mM sodium citrate, pH 3.0.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. The T-cell lymphokine interleukin-26 targets epithelial cells through the interleukin-20 receptor 1 and interleukin-10 receptor 2 chains. Hor S, Pirzer H, Bauer F, de Waal Malefyt R, J Biol Chem 2004 Aug 6;279(32):33343-51 2. Interleukin-20. Xu W, Int Immunopharmacol 2004 May;4(5):627-33 3. Polymorphisms in the interleukin-20 gene: relationships to plaque-type psoriasis. Kingo K, Koks S, Silm H, Genes Immun 2004 Mar;5(2):117-21 4. Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with interleukin-19 and interleukin-20. Pletnev S, Magracheva E, Tobin G, Biochemistry 2003 Nov 4;42(43):12617-24 5. Interleukin 20: discovery, receptor identification, and role in epidermal function. Blumberg H, Conklin D, Grossmann A, Foster D, Parrish-Novak J, Cell 2001 Jan 12;104(1):9-19.