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Interleukin 20, Recombinant, Mouse (IL-20)

Cat no: I8441-53A

Interleukin 20, Recombinant, Mouse (IL-20)

Recombinant Mouse IL-20 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The Recombinant Murine IL-20 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nMouse IL-20 is fully biologically active when compared to standard. The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/mL of recombinant mouse IL-20.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Murine IL-20.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).\n2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Mouse IL-20 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Murine IL-20 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Mouse IL-20 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8441-53A

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

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