Interleukin 22, Recombinant, Human (IL-22, CRF2

Human IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179aa residue protein with a putative 33aa signal peptide that is cleaved to generate a 147aa mature protein that shares ~79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIFa and IL-TIFb, share > 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Ra (previously known as CRF2-4), belonging to the class II cytokine receptor family.\n\nRecombinant Human IL-22 is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 146aa and having a total Mr 33,607D. \n\nSequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Ile-Ser. \n\nBiological Activity: rHuIL-22 is fully biologically active when compared to standard. The Biological Acitivity was determined by the ability to activiate STAT following receptor ligand interaction. \n\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I8443-13B

Size

2ug

Form

Supplied as a lyophilized powder in 40% mannitol, 30% HSA.\nReconstitution: Reconstitute with sterile 18MO-cm H2O to a concentration > 100ug/ml.

Purity

Chromatographically purified ((same/more than) 97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain).\nDimers and Aggregates: < 1% (silver-stained SDS-PAGE gel analysis).\nEndotoxin: < 0.1ng/ug

References

1. Expression of interleukin-22 in murine carcinoma cells did not influence tumour growth in vivo but did improve survival of the inoculated hosts. Nagakawa H, Shimozato O, Takiguchi Y, Scand J Immunol 2004 Nov;60(5):449-54 2. Interleukin 22 (IL-22) plays a protective role in T cell-mediated murine hepatitis: IL-22 is a survival factor for hepatocytes via STAT3 activation. Radaeva S, Sun R, Hong F, Hepatology 2004 May;39(5):1332-42 3. Interleukin-22: a potential immunomodulatory molecule in the lung. Whittington HA, Armstrong L, Millar AB, Am J Respir Cell Mol Biol 2004 Aug;31(2):220-6 4. Comparison of interleukin-22 and interleukin-10 soluble receptor complexes. Logsdon NJ, Jones BC, Cook J, J Interferon Cytokine Res 2002 Nov;22( 11):1099-112 5. Crystal structure of recombinant human interleukin-22. Nagem RA, Colau D, Renauld JC, Structure (Camb) 2002 Aug;10(8):1051-62 6. Interleukin-22 (IL-22) activates the JAK/STAT, ERK, JNK, and p38 MAP kinase pathways in a rat hepatoma cell line. Pathways that are shared with and distinct from IL-10. Lejeune D, Dumoutier L, Kruijer W, J Biol Chem 2002 Sep 13;277(37):33676-82.