Interleukin 3, Recombinant, Human (IL-3)

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases\n\nDescription: \nRecombinant Human IL-3 produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. Recombinant IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRecombinant IL-3 Sf9 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Human IL-3.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.\n\nGene: \nName: IL3\n\nSolubility: \nIt is recommended to reconstitute the lyophilized IL-3 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Human IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant IL-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7663-35C

Size

2ug

Form

Supplied as a lyophilized powder in 0.5X PBS, pH 7.4.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Diphtheria toxin fused to variant human interleukin-3 induces cytotoxicity of blasts from patients with acute myeloid leukemia according to the level of interleukin-3 receptor expression.\n\nBlood 2005 Oct 1;106(7):2527-9\n\n2. Toxicology and pharmacokinetics of DT388IL3, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human interleukin 3 (IL3), in cynomolgus monkeys.\n\nLeuk Lymphoma 2004 Aug;45(8):1647-56\n\n3. [Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy gamma-rays]\n\nZhongguo Wei Zhong Bing Ji Jiu Yi Xue 2004 Jan;16(1):22-5\n\n4. Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system.\n\nProtein Expr Purif 2003 Sep;31(1):34-41\n\n5. Recombinant human interleukin-3 administered concomitantly with chemotherapy in patients with relapsed small cell lung cancer.\n\nJ Exp Ther Oncol 2002 Jan-Feb;2(1):47-52\n\n6. Evidence for a lectin activity for human interleukin 3 and modeling of its carbohydrate recognition domain.\n\nJ Biol Chem 2002 Oct 11;277(41):38764-71