Interleukin 3, Recombinant, Human (IL-3)

Biological Activity: rHuIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1ng/ml, corresponding to a Specific Activity of 1x107 IU/mg.\n\nAA Sequence:\nAPMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-35D

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Effect of allergen-specific immunotherapy on recombinant human interleukin 3-mediated amplification of allergen-induced basophil histamine release.\nAllergy Asthma Proc 2005 Nov-Dec;26(6):456-62\n2. Secretion of functional human interleukin-3 from Bacillus subtilis.\nJ Biotechnol 2006 May 17;123(2):211-24\n3. Diphtheria toxin fused to variant human interleukin-3 induces cytotoxicity of blasts from patients with acute myeloid leukemia according to the level of interleukin-3 receptor expression.\nBlood 2005 Oct 1;106(7):2527-9\n4. Toxicology and pharmacokinetics of DT388IL3, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human interleukin 3 (IL3), in cynomolgus monkeys.\nLeuk Lymphoma 2004 Aug;45(8):1647-56\n5. [Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy gamma-rays] Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2004 Jan;16(1):22-5\n6. Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system.\nProtein Expr Purif 2003 Sep;31(1):34-41