Interleukin 3, Recombinant, Mouse (IL-3)

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. \n\nBiological Activity: Murine IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine M-NFS-60 cells is < 0.05ng/ml, corresponding to a Specific Activity of 2x107IU/mg.\n\nAA Sequence:\nMDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-36B

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE.

References

1. Constitutive activation of JAK2 confers murine interleukin-3-independent survival and proliferation of BA/F3 cells. J Biol Chem 1999 Mar 5;274(10):6342-9\n2. The diphtheria toxin transmembrane domain as a pH sensitive membrane anchor for human interleukin-2 and murine interleukin-3. Protein Eng 1998 Nov;11(11):1111-20\n3. Homodimeric murine interleukin-3 agonists indicate that ligand dimerization is important for high-affinity receptor complex formation. Growth Factors 1994;10(1):17-27\n4. Stem cell responses in myelosuppressed mice following sequential treatment with recombinant human interleukin 1 (rHuIL-1), recombinant murine interleukin 3 (rMuIL-3) and recombinant human macrophage colony-stimulating factor (rHuM-CSF). Stem Cells 1994 Jan;12(1):103-13\n5. The c-kit receptor transduces the stem cell factor-triggered growth signal in murine interleukin-3-dependent cell line. Cytotechnology 1994;16(1):27-35\n6. Retroviral-mediated transfer of the murine interleukin-3 gene engineered for intracellular retention results in a myeloproliferative syndrome but is associated with circulating interleukin-3 levels. Exp Hematol 1993 Aug;21(9):1245-54