Interleukin 3, Recombinant, Rat (IL-3)

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases\n\nDescription: \nRecombinant Rat IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 15783 Dalton. Recombinant Rat IL-3 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nrrIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine MC-9 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 1 x 10e5 IU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Rat IL-3.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of Rat IL-3 as a Reference Standard.\n\nGene: \nName: Il3; Synonyms: Il-3\n\nSolubility: \nIt is recommended to reconstitute the lyophilized IL-3 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Rat IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7663-36R

Size

2ug

Form

Supplied as a lyophilized powder in 5mM sodium citrate, pH 4.0.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Cell cycle kinetic changes induced by interleukin-3 and interleukin-6 during ex vivo expansion of mobilized peripheral blood CD34 cells.\n\nHaematologica 2006 Jan;91(1):121-4\n\n2. Interleukin-3 (IL-3) is overexpressed by T lymphocytes in multiple myeloma patients.\n\nBlood 2006 Jan 15;107(2):841-2\n\n3. Effects of a leukemia-associated gain-of-function mutation of SHP-2 phosphatase on interleukin-3 signaling.\n\nJ Biol Chem 2006 Mar 3;281(9):5426-34\n\n4. Interleukin-3, -5, and granulocyte macrophage colony-stimulating factor induce adhesion and chemotaxis of human eosinophils via p38 mitogen-activated protein kinase and nuclear factor kappaB.\n\nImmunopharmacol Immunotoxicol 2005;27(3):371-93\n\n5. S6 kinase 2 potentiates interleukin-3-driven cell proliferation.\n\nJ Leukoc Biol 2005 Dec;78(6):1378-85\n\n6. Low-dose cytosine arabinoside (LD-AraC) vs LD-AraC plus granulocyte/macrophage colony stimulating factor vs LD-AraC plus Interleukin-3 for myelodysplastic syndrome patients with a high risk of developing acute leukemia: final results of a randomized phase III study (06903) of the EORTC Leukemia Cooperative Group.\n\nLeukemia 2005 Nov;19(11):1929-33