Interleukin 33 (IL-33)

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a member of IL-1 cytokine family. IL-33 is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 Alpha or Beta in arterial smooth muscle, dermal fibroblasts, and keratinocytes. IL-33 is synthesized as a 270 aa, 32 kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18 kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature human IL-33 shares 55% aa identity with mouse IL-33. \n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions\nImmunocytochemistry: 8-25ug/ml detects human IL-33 in paraformaldehyde-fixed human PBMCs.\nDirect ELISA: 0.5-1ug/ml The detection limit is ~5ng/well\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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