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Interleukin 33, Recombinant, Human (IL-33)

Cat no: I7663-97E

Interleukin 33, Recombinant, Human (IL-33)

IL-33 is a member of the IL-1cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells. Recombinant human IL-33 is anon-glycosylated protein, consisting of 160aa and having a total molecular weight of 18.1kD.\n\nRecombinant protein corresponding to human IL-33, expressed in E. coli\n\nBiological Activity: \nThe activity is determined by the ability to induce the proliferation of D10.G4.1 cells co-stimulated with anti-CD3 and is typically 0.06-0.24ng/ml.\n\nAmino Acid Sequence:\nMSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA.. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-97E

Size

2ug

Form

Supplied as a lyophilized powder from PBS, pH 7.5. \nReconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 0.1mg/ml. Endotoxin: (same/less than)0.1EU/ug (LAL)

Purity

(same/more than)95% as determined by reducing and non-reducing SDS-PAGE

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