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Interleukin-33, Recombinant, Human (IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor from High Endothelial Venules, NF-HEV, IL33, C9orf26, IL1F11, NFHEV)

Cat no: I7663-97Q

Interleukin-33, Recombinant, Human (IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor from High Endothelial Venules, NF-HEV, IL33, C9orf26, IL1F11, NFHEV)

Interleukin 33 (IL-33) is a proimflammatory cytokine belonging to the IL-1 family. IL-33 is cleaved in vivo, generating an N-terminal and a C-terminal fragment. The C-terminal fragment corresponds to the mature IL-33 protein and mediates its biological effect by interacting with the IL-1 receptor, also known as ST2. Binding of mature IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces the production of associated cytokines in particular IL-4, IL-5 and IL-13 causing severe pathological changes in mucosal organs.\n\nSource: \nRecombinant corresponding to aa112-270 from C-terminal Interleukin-33 fragment coding sequence, fused to 6x His-tag at N-terminal, expressed in E. coli.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Dot Blot, Immunohistohemistry, Immunoassays, Development of Rapid tests and other immunoassay. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-97Q

Size

10ug

Applications

ELISA, IHC, WB

Form

Supplied as liquid.

Purity

~95% (SDS-PAGE)

References

1. Schmitz J. et al. (2005), Immunity. 23(5): 479-90.

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