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Interleukin 4, Human (IL-4) BioAssay(TM) ELISA Kit

Interleukin 4, Human (IL-4) BioAssay(TM) ELISA Kit

Cat no: I8426-01

Supplier: United States Biological
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Principle: This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitate the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
Catalogue number: I8426-01
Applications: ELISA
Size: 96Tests
References: 1. Howard, M. et al.(1982) J. Exp. Med. 155:914 2. Vitetta, E. S. et al.(1985) J. Exp. Med. 162:1726 3. Park, L. S. et al.(1987) J. Exp. Med. 166:476 4. Estes, M. L. et al.(1993) Am. J. Pathol. 143:337 5. Lacey, D. L. et al. (1993) J. Cell. Biochem. 53:122 6. Cabrillant, H. et al. (1987) Biochem.Biophys. Res. Commun. 149: 995 7. Ohara, J. et al. (1987)Nature 325: 537 8. Lowenthal, J. W. et al. (1988) J. Immunol. 140:456 9. Mosmann, T. R. et al. (1991) Immunol. Today 12:A49 10. del Prete, G. et al. (1994) Lab Invest. 70:299 11. Sideras, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:218 12. Seder, R. A. et al. (1992) J. Immunol. 148:1652 13. Braddig, P. et al. (1992) J. Exp. Med. 176:1381 14. MacGlashan, D. et al. (1994) J. Immunol. 152:3006 15. Paul, W. E. et al. (1991) Blood 77:1859 16. Banchereau, J. et al (1994) "Interleukin 4": in The Cytokine Handbook, 2nd ed. , A. Thomson, Ed. Academic Press, New York, p. 9917. Carr. C. et al. (1991)

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