Interleukin 4, Recombinant, Mouse (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pit

Recombinant Murine IL-4 is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13.5kD.\n\nAA Sequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ile-His-Gly. \n\nBiological Activity:\nFully biologically active when compared to standard. The ED50 as determined by the dose-dependent prolifiration of Murine HT-2 cells is less then 2ng/ml, corresponding to a Specific Activity of 5x10e6 IU/mg. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8426-10A

Size

2ug

Form

Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O, 0.1% BSA or HSA to > 0.1mg/ml.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: (same/less than) 0.1ng/ug (IEU/ug).

References

1. Modified vaccinia virus Ankara immunization protects against lethal challenge with recombinant vaccinia virus expressing murine interleukin-4. McCurdy LH, Rutigliano JA, Chen M, J Virol 2004 Nov;78(22):12471-9 2. Alternative splicing of murine interleukin-4 mRNA. Yatsenko OP, Filipenko ML, Khrapov EA, Bull Exp Biol Med 2004 Feb;137(2):179-81 3. A murine interleukin-4-Ig fusion protein regulates the expression of Th1- and Th2-specific cytokines in the pancreas of NOD mice. Walz M, Overbergh L, Kolb H, Horm Metab Res 2002 Oct;34(10):561-9 4. Recombinant herpes simplex virus type 1 expressing murine interleukin-4 is less virulent than wild-type virus in mice. Ghiasi H, Osorio Y, Nesburn AB, J Virol 2001 Oct;75(19):9029-36 5. Immune gene therapy of experimental mouse brain tumor with adenovirus-mediated gene transfer of murine interleukin-4. Yoshikawa K, Kajiwara K, Uchida T, Cancer Immunol Immunother 2000 Apr;49(1):23-33 6. Combined therapy of B16(F10) murine melanoma using E. coli cytosine deaminase gene and murine interleukin-4 gene. Missol-Kolka E, Sochanik A, Neoplasma 1998;45(5):305-11.