Interleukin 5, Recombinant, Human (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Interleukin-5 (IL-5) is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils.Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant human IL-5 is a 26kD disulfide-linked homodimeric protein containing two 116 amino acid chains.\n\nBiological Activity: Human IL-5 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent proliferation of TF-1 cells is (same/less than) 0.15ng/ml, corresponding to a specific activity of (same/more than) 6x10e6 units/mg. For most in vitro applications, IL-5 exerts its biological activity in the concentration range of 0.1-10ng/ml.\n\nResponding Cells (partial list): B cells, eosinophils\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at \n-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: USA

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SPECIFICATIONS

Catalog Number

I8427

Size

2ug

Form

Supplied as a lyophilized powder without additives.\nReconstitution: Reconstitute with sterile water to a concentration of 0.1-1mg/ml.

Purity

Highly Purified ((same/more than) 98% by SDS-PAGE)\nEndotoxin: (same/more than) 0.1ng/ug

References

1. van Beek, J.: Decay-accelerating factor (CD55) is expressed by neurons in response to chronic but not acute autoimmune central nervous system inflammation associated with complement activation. Journal of Immunology 174(4): 2353-65 (2/15/2005). 2. Kulka, M.: High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression. Blood; 105(2): 592-9 (1/15/2005). 3. Ferland, C.: IL-16 activates plasminogen-plasmin system and promotes human eosinophil migration into extracellular matrix via CCR3-chemokine-mediated signaling and by modulating CD4 eosinophil expression. Journal of Immunology 173(7): 4417-24 (10/1/2004). 4. Ling, Lin: Active Inhibition of Plasma Cell Development in Resting B Cells by Microphthalmia-associated Transcription Factor. J. Exp. Med. 200(1): 115-122 (7/5/2004). 5. Akseli Hemminki: Modulation of coxsackie-adenovirus receptor expression for increased adenoviral transgene expression. Cancer Research 63: 847-853 (2/15/2003). 6. Ryutaro Hirasawa: Essential and instructive roles of GATA factors in eosinophil development. J. Exp. Med. 195(11): 1379-1386 (6/3/2002). 7. Fabrice Piu: Dissection of the cytoplasmic domains of cytokine receptors involved in STAT and Ras dependent proliferation. Oncogene 21(22): 3579-3591 (5/16/2002). 8. Qing Ge: Homeostatic T cell proliferation in a T cell-dendritic cell coculture system. PNAS 99(5): 2983-2988 (3/5/2002). 9. Vinante, F.: Granulocyte-Macrophage Colony-Stimulating Factor Induces Expression of Heparin-Binding Epidermal Growth Factor-Like Growth Factor Diptheria Toxin Receptor and Sensitivity to Diptheria Toxin in Human Neutrophils. Blood 94(9): 3169-3177 (11/1/1999). 10. Liu, L.: Triple Role of Platelet-Activating Factor in Eosinophil Migration Across Monolayers of Lung Epithelial Cells: Eosinophil Chemoattractant and Priming Agent and Epithelial Cell Activator1. The Journal of Immunology 161: 3064-3070 (1/1/1998).