Interleukin 5, Recombinant, Human (Interleukin-5, IL5, IL-5, B-cell Differentiation Factor I, Eosinophil Differentiation Factor, EDF, T-cell Replacing Factor, TRF)

Source:\nRecombinant corresponding to 132aa of human Interleukin-5 expressed in E.coli is a dimeric, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~26.5kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells was foundto be less then 0.15ng/ml, corresponding to a Specific Activity of 6x10 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.625 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-5 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8427-01D

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. The conserved lymphokine element 0 is a powerful activator and target for corticosteroid inhibition in human interleukin-5 transcription. Growth Factors 2005 Sep;23(3):211-21. 2. Cytokine recognition by human interleukin 5 receptor. Vitam Ho rm 2005;71:321-44. 3. Human interleukin-5 expression is synergistically regulated by histone acetyltransferase CBP/p300 and transcription factors C/EBP, NF-AT and AP-1. Cytokine 2004 Aug 21-Sep 7;27(4-5):93-100. 4. Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance. Anal Biochem 2004 Apr 15;327(2):165-75. 5. Kinetic interaction analysis of human interleukin 5 receptor alpha mutants reveals a unique binding topology and charge distribution for cytokine recognition. J Biol Chem 2004 Mar 5;279(10):9547-56. 6. Sequence-specific NMR resonance assignments for human interleukin-5. J Biomol NMR 2002 Aug;23(4):329-30.

Alternative Names

Interleukin 5 (Colony-stimulating Factor, Eosinophil)