Interleukin 5, Recombinant, Rat (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Interleukin-5 (IL5), also known as eosinophil differentiation factor (EDF), is a lineage-specific cytokine for eosinophilpoiesis . It regulates eosinophil growth and activation, and thus plays an important role in diseases associated with increased levels of eosinophils, including asthma. IL5 has a similar overall fold to other cytokines (e.g., IL2, IL4 and GCSF), but while these exist as monomeric structures, IL5 is a homodimer. The fold contains an anti-parallel 4-alpha-helix bundle with a left handed twist, connected by a 2-stranded anti-parallel beta-sheet . The monomers are held together by 2 interchain disulfide bonds.\n\nDescription: \nRecombinant Rat IL-5 produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13074 Dalton. The Recombinant Rat IL-5 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRat IL-5 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of BCL-1 cells was found to be < 0.15ng/ml.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Rat IL-5.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.635 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IL-5 as a Reference Standard.\n\nGene: \nName: Il5; Synonyms: Il-5\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Rat IL-5 in sterile 18M -cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Rat IL-5 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-5 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8437-06A

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Interleukin-5 (IL-5) augments the progression of liver fibrosis by regulating IL-13 activity.\n\nInfect Immun 2006 Mar;74(3):1471-9\n\n2. Clinical significance of serum Th1-, Th2- and regulatory T cells-associated cytokines in adult T-cell leukemia/lymphoma: high interleukin-5 and -10 levels are significant unfavorable prognostic factors.\n\nInt J Cancer 2006 Jun 15;118(12):3054-61\n\n3. [The role of interleukin-5 in manifestation of bronchial hyperreactivity (an experimental study)]\n\nPatol Fiziol Eksp Ter 2005 Oct-Dec;(4):20-2\n\n4. Pelvic irradiation-induced eosinophilia is correlated to prognosis of cervical cancer patients and transient el evation of serum interleukin 5 level.\n\nRadiat Med 2005 Aug;23(5):317-21\n\n5. Interleukin-9 stimulates the production of interleukin-5 in CD4+ T cells.\n\nEur Cytokine Netw 2005 Sep;16(3):233-9\n\n6. Dexamethasone suppresses human interleukin-5 gene promoter.\n\nBull Exp Biol Med 2005 Jul;140(1):80-2