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Interleukin 6, Recombinant, Human (IL-6)

Cat no: I8428-05

Interleukin 6, Recombinant, Human (IL-6)

Recombinant human IL-6 is a 20.5kD protein containing 184 amino acid residues. \n\nBiological Activity: ED50 as determined in a IL-6 dependent cell proliferation assay using 7TD1 cells is (same/less than) 0.1ng/ml, which corresponds to a speci?c activity (same/more than) 1x10e7units/mg. \n\nStorage and Stability: \nLyophilized: Stable for 2 years at 4 degrees C. \nReconstituted: Solutions (same/more than) 100ng/ul may be stored for 3 months at -20 degrees C without detectable loss of activity. Less concentrated solutions stored at -20 degrees C are less stable. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer with 0.5% BSA.

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SPECIFICATIONS

Catalog Number

I8428-05

Size

25ug

Form

Supplied as a lyophilized powder from 10mM acetic acid, carrier free. Filtered through a 0.2um membrane and packaged aseptically.\nReconstitution: Initially, rehydrate in 10mM acetic acid to a concentration of 0.1-0.5ug/ml. Note: If the initial rehydration is too dilute, activity may be lost due to non-speci?c adsorption to the container.

Purity

(same/more than) 95%\nEndotoxin: (same/less than) 0.1ng/ug

References

1. Akina, S., et al., Advances Immunol. 54: 1, 1993. 2. Yamasaki, K., et al., Science 241: 825, 1988. 3. Sugita, T., et al., J. Exp. Med. 171: 2001, 1990. 4. Hibi, M., et al., Cell 63: 1149, 1990. 5. Saito, M., et al., J. Immunol. 148: 4066, 1992. 6. Murakami, M., et al., Science 260: 1808, 1993. 7. Lotz, M., et al., J. Exp. Med. 167: 1253, 1988. 8. Tosato, G. and Pike, S.E., J. Immunol. 141: 1556, 1988. 9. Uttenhove, C., et al., J. Exp. Med. 167: 1417, 1988. 10. Kitamura, T., et al., J. Cell. Physiol. 140: 323, 1989.

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