Interleukin 6, Recombinant, Human (IL-6)

Recombinant Human IL-6 is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21kD.\n\nSequence:\nMet-Pro-Val-Pro-Pro (first 5 N-terminal aa) \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\nrHuIL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine 7TD1 cells is less then 0.1ng/ml, corresponding to a Specific Activity of ~1 x 10e6 IU/mg. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding ddH2O, 0.1% HSA or BSA and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8428-05A

Size

5ug

Form

Supplied as a lyophilized powder in 1.6% HSA and 2% mannitol.\nReconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of (same/more than) 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: (same/less than) 0.1ng/ug (IEU/ug)

References

1. Saha, A., et al., Eur. J. Immunogenet. 30(6): 397-400 (2003). 2. Matthews, V., et al., J. Biol. Chem. 278(40): 38,829-38,839 (2003). 3. Li, Y., et al., FEMS Immunol. Med. Microbiol. 37(2-3): 135-145 (2003). 4. Varghese, J.N., et al., Proc. Natl. Acad. Sci. USA 99(25): 15,959-15,964 (2002). 5. Song, J., et al., J. Med. Virol. 68(3): 404-411 (2002). 6. Li, Y., et al., Protein Expr. Purif. 25(3): 437-447 (2002).