Interleukin 6, Recombinant, Rat (IL-6)

Source: \nRecombinant corresponding to rat Interleukin 6 expressed in E. coli.\n\nMolecular Weight: \n~22kD\n\nEndotoxin: (same/less than)0.1ng/ug\n\nActivity:\nED50 =0.03-0.1ng/ml. The biological activity is determined by measuring the dose dependent proliferation of IL-6 dependent B9 cells. A concentration range of 0.1 to 10.0ng/ml is effective for most in vitro applications. The optimal concentration for each specific application should be determined by an initial dose response assay.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8428-15A

Size

10ug

Form

Supplied as a lyophilized powder. BSA free. Reconstitute with sterile 100mM acetic acid.

Purity

~95% (SDS-PAGE)

References

1. Northemann, W., T.A. Braciak, M. Hattori, F. Lee, and G.H. Fey (1989) Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells. J. Biol. Chem. 264:16072-16082. 2. Aarden, L.A., E.R. De Groot, O.L. Schaap, and P.M. Landsdorp (1987) Production of hybridoma growth factor by human monocytes. Eur. J. Immunol. 17:1411-1416. 3. Hirano, T. (1994) In: The Cytokine Handbook, 2nd Edition (ed. A. Thompson) Academic Press Limited, San Diego, CA, pp. 145-168. 4. Rees, G.S., C. Ball, H.L. Ward, C.K. Gee, G. Tarrant, Y. Mistry, S. Poole, and A.F. Bristow (1999) Rat interleukin 6: expression in recombinant Escherichia coli, purification and development of a novel ELISA. Cytokine 11:95-103. 5. Ikeda, Y., A. Sugawara, Y. Taniyama, A. Uruno, K. Igarashi, S. Arima, S. Ito, and K. Takeuchi (2000) Suppression of rat thromboxane synthase gene transcription by peroxisome proliferator-activated receptor in macrophages via an interaction with NRF2. J. Biol. Chem. 275(42):33142-33150.